Andrea Dovizioso led for Ducati the field in FP1 at the Gran Premio d'Italia Oakley at Mugello, taking first blood for Ducati ahead of Ducati Team test rider Michele Pirro.
Le Mans winner Maverick Vinales was third quickest as Ducati and Yamaha locked out the top eight.
FP1 Result:
| Pos. | Rider | Team | Bike | Km/h | Time | Gap 1st/Prev. |
| 1 | Andrea DOVIZIOSO | Ducati Team | Ducati | 352.3 | 1'47.394 | |
| 2 | Michele PIRRO | Ducati Team | Ducati | 351.4 | 1'47.595 | 0.201 / 0.201 |
| 3 | Maverick VIÑALES | Movistar Yamaha MotoGP | Yamaha | 343 | 1'47.669 | 0.275 / 0.074 |
| 4 | Johann ZARCO | Monster Yamaha Tech 3 | Yamaha | 338.1 | 1'47.674 | 0.280 / 0.005 |
| 5 | Hector BARBERA | Reale Avintia Racing | Ducati | 351.4 | 1'47.708 | 0.314 / 0.034 |
| 6 | Jorge LORENZO | Ducati Team | Ducati | 343.5 | 1'47.747 | 0.353 / 0.039 |
| 7 | Danilo PETRUCCI | OCTO Pramac Racing | Ducati | 351.2 | 1'47.962 | 0.568 / 0.215 |
| 8 | Jonas FOLGER | Monster Yamaha Tech 3 | Yamaha | 338.9 | 1'47.974 | 0.580 / 0.012 |
| 9 | Marc MARQUEZ | Repsol Honda Team | Honda | 340.3 | 1'47.980 | 0.586 / 0.006 |
| 10 | Cal CRUTCHLOW | LCR Honda | Honda | 340.3 | 1'48.194 | 0.800 / 0.214 |
| 11 | Andrea IANNONE | Team SUZUKI ECSTAR | Suzuki | 350.1 | 1'48.274 | 0.880 / 0.080 |
| 12 | Alvaro BAUTISTA | Pull&Bear Aspar Team | Ducati | 348 | 1'48.289 | 0.895 / 0.015 |
| 13 | Dani PEDROSA | Repsol Honda Team | Honda | 341.7 | 1'48.291 | 0.897 / 0.002 |
| 14 | Aleix ESPARGARO | Aprilia Racing Team Gresini | Aprilia | 339.1 | 1'48.394 | 1.000 / 0.103 |
| 15 | Valentino ROSSI | Movistar Yamaha MotoGP | Yamaha | 340.9 | 1'48.513 | 1.119 / 0.119 |
| 16 | Scott REDDING | OCTO Pramac Racing | Ducati | 345.4 | 1'48.743 | 1.349 / 0.230 |
| 17 | Jack MILLER | EG 0,0 Marc VDS | Honda | 340.9 | 1'49.119 | 1.725 / 0.376 |
| 18 | Tito RABAT | EG 0,0 Marc VDS | Honda | 344.3 | 1'49.123 | 1.729 / 0.004 |
| 19 | Loris BAZ | Reale Avintia Racing | Ducati | 335.4 | 1'49.526 | 2.132 / 0.403 |
| 20 | Pol ESPARGARO | Red Bull KTM Factory Racing | KTM | 341.4 | 1'49.677 | 2.283 / 0.151 |
| 21 | Sam LOWES | Aprilia Racing Team Gresini | Aprilia | 336.4 | 1'49.679 | 2.285 / 0.002 |
| 22 | Karel ABRAHAM | Pull&Bear Aspar Team | Ducati | 345.1 | 1'49.754 | 2.360 / 0.075 |
| 23 | Bradley SMITH | Red Bull KTM Factory Racing | KTM | 336.8 | 1'50.686 | 3.292 / 0.932 |
| 24 | Sylvain GUINTOLI | Team SUZUKI ECSTAR |